DNA

Part:BBa_K2384004:Design

Designed by: Size Cai   Group: iGEM17_FAFU-CHINA   (2017-10-21)


DsbA+MBP(Hg)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 255
    Illegal BglII site found at 267
    Illegal BglII site found at 711
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is more suitable for expression in Bacillus megaterium.This coding sequence(basic parts) is synthesized without terminator codon.


Source

Our sequence optimization is based on the sequence provided by Peking University's IGEM WIKI in 2010. This gene is composed of synthetic biology company.

References